Please note that antibodies should be stored in a 80c freezer if they arrive frozen. Vimentin is a strong arterial substrate for transglutaminases. Antivimentin antibody rv202 cytoskeleton marker ko. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Mice lacking vimentin develop and reproduce without an. Genetex currently offers a continuously expanding catalog of reagents designed expressly for zebrafish research, with more than 400 newly developed zebrafish antibodies. Myc and fgf are required for zebrafish neuromast hair cell. Antibodies to vimentin can be used to study sarcoma tumor markers. Confocal microscopy of whole fish immunofluorescence with vimentin antibody highlights metastatic melanoma cells and their location within the zebrafish. A monoclonal antibody stains radial glia in the adult zebrafish danio rerio cns.
Vimentin v9 is a mouse monoclonal antibody raised against purified vimentin from eye lens of porcine origin. Vimentin was detected in immersion fixed k562 human chronic myelogenous leukemia cell line but is not detected in vimentin knockout ko k562 human cell line cell line using goat antihuman vimentin antigen affinitypurified polyclonal antibody. Please use this form to recommend updates to the information in zfin. In the embryonic nervous system, however, vimentin. The development of f1 zebrafish produced by the parent fish exposed to mclr is shown in fig. The fate of human malignant melanoma cells transplanted. Transglutaminasemediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. All together, these data support a novel mechanism by which vimentin interferes with a mirdependent negative regulation of tf mrna, thereby promoting coagulant activity. The intermediate filament network changes from being composed of vimentin to.
Lowpower immunofluorescence image of a 24h zebrafish embryo tail somites 1830 using anti. In this study igm antibody was detected but not igg antibodies and none of the patients had ava. Vimentin is a 54 kda nucleus proteincoding gene expressed in mesenchymal cells. The zebrafish book a guide for the laboratory use of. Vimentin can be phosphorylated by akt1, a kinase activated downstream of ras and pi3k, and phosphorylation of vimentin protects it from caspaseinduced proteolysis and enhances cell migration and metastasis. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate. Its name comes from the latin vimentum which refers to an array of flexible rods. Access journals video booksebooks trade magazines enewsletters. Zebrafish antibodies primary antibodies sigmaaldrich. Vinculin b deficiency causes epicardial hyperplasia and coronary. Some distinctive features of zebrafish myogenesis based on. Wash three times for 5 min each with 15 ml of tbst. Sectioning of embryos or larvae staining sections with antibodies using pap.
Compared to the fish in the control group, the f1 zebrafish were prematurely hatched at 48. A monoclonal antibody stains radial glia in the adult zebrafish. Vimentin, also known as ctrct30, is the full name of vim. Incubate membrane and primary antibody at the appropriate dilution and diluent as recommended in the product datasheet in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4c. Due to their striking genomic similarity to humans, regenerative. Abcam antibodies and reagents supplier, find any antibody. A guide for the laboratory use of zebrafish danio rerio. Along with actin microfilaments and tubulin microtubules, vimentin is the third major constituent of the cytoskeleton. Vimentin staining confirms mesenchymal origin of some tumors. Islands of hn2 cells form after 4 days in culture forming beautiful flower like structures.
Vimentin antibody detects endogenous levels of total human vimentin. Using a zebrafish lateral line neuromast hair cell regeneration model, we show. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in. Broad interspecies crossreactivity has been observed with human, rat, and chicken vimentin. As an organizer of a number of critical proteins, vimentin function involved in attachment, migration, and cell signaling. Role of antivimentin antibodies in allograft rejection. E nvclbegfp fusion protein expression detected with a gfp antibody in. Myc and fgf are required for zebrafish neuromast hair cell regeneration. I am planning on using mouse monoclonal vimentin antibody 2d1, nbp192687 on mouse fibroblasts. The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the specific staining patterns obtained with particular clones. The hybridomas are stored at the monoclonal antibody.
This antibody recognizes human, mouse, rat antigen. Vimentins dynamic structural changes and spatial reorganization in response to extracellular stimuli helps to coordinate various signaling pathways 4. Ma511883 targets vimentin in ihc p applications and shows reactivity with bovine, canine, chicken, equine, feline, gerbil, hamster, human, mouse, nonhuman primate, porcine, rabbit, and rat samples. Antivimentin antibody v9 cytoskeleton marker alexa fluor 488 ab195877 specific references 5. We use cookies to enhance the usability of our website. Global supplier of antibodies, proteins and elisa kits 25 years expertise in serving research and diagnostic customers.
This antibody recognizes human, mouse, and rat antigen. Human vimentin is a 53,521 dalton protein 466 amino acids expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. Microcystinlr promotes zebrafish danio rerio oocyte in. The kit contains enough primary and secondary antibody. Libraries of zebrafishspecific antibodies are now available, but few. The vimentin antibody from proteintech is a mouse monoclonal antibody to a recombinant protein of human vimentin. The vimentin antibody sampler kit provides an economical means to detect total levels of vimentin, vimentin phosphorylated at ser56, and vimentin phosphorylated at ser82. Wb of crude extract of human embryonic kidney stained with vimentin, showing a single strong clean band at 50kda and view of mixed neuronglial cultures stained with vimentin green and gfap antibody ra22101, red. Also, current batches of zirc antibody may have dilution rates that vary from the dilution rates found in publications. Compare anti vimentin antibody products from leading suppliers on biocompare.
Vimentin is a developmentally regulated intermediate filament protein ifp found in cells of mesenchymal origin. Intermediate filamentents, along with microtubules and actin microfilaments, make up the. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the pns, and by neural stem cells of the cns. The vimentin antibody has been validated for the following applications.
Vimentin is an intermediate filament protein, expressed exclusively by mesenchymal cells. A dominant vimentin variant causes a rare syndrome with. Myc and fgf are required for zebrafish neuromast hair cell plos. We thank dr hans heid and professor harald herrmann german cancer research center, heidelberg, germany for providing antiperilipin antibody and human vimentin cdna.
The exact position of epitope sequence recognized by anti vimentin antibody clone rv202 is not determined however, the antibody must recognize an evolutionary highly conserved epitope. B the 3d larvae labeled with antiacetylated tubulin antibody. The ma511883 immunogen is purified vimentin from pig eye lens. Arg66199 antivimentin antibody sqab1721 iccif image. Vimentin intermediate filament rings deform the nucleus during the first steps of adhesion. Vimentin antibody nbp27 whole cell extracts 30 ug was separated by 10% sdspage, and the membrane was blotted with vimentin antibody at a dilution of 1.
Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio. Vimentin antibody staining protocol for immunohistochemistry. The kit contains enough primary and secondary antibody to perform two western blot experiments. Vimentin antibody protein level used designations for anti vimentin vim antibodies vimentin, vimentin, vimentin 1, vimentin 12, classiii intermediate microfilament gene id species.
Hela cells were fixed with 4% paraformaldehyde for 30 min at rt, permeabilized with 0. Vimentin prevents a mirdependent negative regulation of. Vimentin antibody rabbit polyclonal from proteintech validated in western blot wb, immunohistochemistry ihc, immunofluorescence if, flow cytometry fc, enzymelinked immunosorbent assay elisa applications. Antihucd is a rabbit polyclonal antibody that stains inner retinal. Therefore, vimentin is critical for proper organell anchoring and cellular structure. Your search returned 2096 vimentin antibodies across 61 suppliers. Oct 29, 2012 it was initially shown in 2001 that heart transplant patients produce igm anti vimentin antibodies within 2 years of transplantation and that higher titres in the first two years were predictive of cardiac allograft vasculopathy cav at 5 years. The vimentin v9 antibody from santa cruz biotechnology, inc. Further information about each individual vimentin antibody. Immunohistochemistry of wholemount and sectioned zebrafish tissue. Vimentin specificity is shown by immunocytochemistry in knockout cell line. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. A 28h wholemount zebrafish embryo labeled with an antibody which recognizes the transcription factor, pax6 brown label showing a lateral view with anterior to the left. Vimentin elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples.
Wildtype wt and vimentin knockout ko 293t cell extracts 30. The zebrafish book, in a guide for the laboratory use of zebrafish danio. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Ovarian and uterine corpus endometrioid adenocarcinomas have different patterns of vimentin expression. If you continue, well assume that you are happy to receive all cookies. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Is expressed in several structures, including axis. Among all these vimentin antibodies, there are 2 anti vimentin rabbit monoclonal antibodies, 3 anti vimentin rabbit polyclonal antibodies. Hardcopies of the 4th edition of the zebrafish book can be obtained for. Vim antibody mouse anti human vimentin antibody prospec. Vimentin antibody v9 scbt santa cruz biotechnology. Vimentin is a structural protein that in humans is encoded by the vim gene.
Further information about each individual vimentin antibody is available by following the associated links. Mouse vimentin antibody for immunostaining was from sigma v5255. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Phosphorylation of vimentin at ser56 in smooth muscle cells regulates structural arrangement of vimentin filaments in response to serotonin 5,6. All the vimentin anbodies are produced in house and all are in stock. Immunofluorescence staining of hela cells with antibody to reveal vimentin containing intermediate filaments in green and antibody. The exact position of epitope sequence recognized by anti vimentin antibody clone rv202 is not determined however, the antibody must recognize an evolutionary highly conserved epitope, since it shows a broad spectrum of species cross reactivity from human to zebrafish. There are 3 vimentin antibody for wb, 4 vimentin antibody for ihcp. For western blots, incubate membrane with diluted primary antibody in 5% wv nonfat dry milk, 1x tbs, 0. Adult zebrafish were subjected to ouabainmediated lesion that. Has the antibody been tested for crossreactivity with endogenous igg. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. We report a zebrafish genetrap vinculin b vclb mutant that displays abnormal.
Sep 08, 2016 it was found that cells without vimentin are extremely delicate when disturbed with a micropuncture. Ihc staining of zebrafish for cytokeratin is impacted by fixative or length of. This monoclonal antibody reacts with the intermediate filament protein vimentin present in cells of mesenchymal origin. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Evaluating the effects of various decalcification protocols on. Vimentin antibody customerized service is available. If thawed upon arrival, please keep antibodies in the refrigerator at 4c. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system. Some steps, like fusion before striation, seem to be different in the zebrafish when compared. The fate of human malignant melanoma cells transplanted into.